DREAM represses distinct targets by cooperating with different THAP domain proteins

DREAM represses distinct targets by cooperating with different THAP domain proteins

Blog Article

Summary: The DREAM (dimerization partner [DP], retinoblastoma [Rb]-like, E2F, and MuvB) complex controls cellular quiescence by repressing cell-cycle and other genes, but its mechanism of action is unclear.Here, we demonstrate that two C.elegans THAP domain proteins, LIN-15B and LIN-36, co-localize with DREAM and function by different mechanisms for repression of distinct sets of targets.LIN-36 represses classical Model Truck cell-cycle targets by promoting DREAM binding and gene body enrichment of H2A.

Z, and we find that DREAM subunit EFL-1/E2F is specific for LIN-36 targets.In contrast, LIN-15B represses Filters germline-specific targets in the soma by facilitating H3K9me2 promoter marking.We further find that LIN-36 and LIN-15B differently regulate DREAM binding.In humans, THAP proteins have been implicated in cell-cycle regulation by poorly understood mechanisms.

We propose that THAP domain proteins are key mediators of Rb/DREAM function.